Services development
On receipt of a request, a qualified bioanalytical chemist from OWL Genomics will contact the client to discuss the details of the project and prepare a quotation.
Experimental Planning
Expert advice is offered for optimal sample collection, sample preparation procedures, analytical methodology and data processing methods to provide a high quality, fast turnaround, cost efficient final product.
Metabolite Extraction / Sample Preparation
Depending upon the nature of the project in question, a series of validated metabolite extraction techniques are available for a wide range of biofluids and tissues. An optimal choice of method will be made, depending on the medium and the section of the metabolome considered to be of prime interest (e.g. lipids, amino-acids, etc).
Analytical Platform
Samples are typically analysed in positive and negative electrospray ionisation (ESI) modes. Accurate mass is assured for all ions detected, whilst a range of MS/MS possibilities (including rapid switching between low and high collision energies -MSE) provide powerful fragmentation analysis tools for compound identification.
The analytical platform at OWL Genomics includes:
- UPLC/MS: OWL Genomics has acquired state of the art mass spectrometers, that interface to ultra performance liquid chromatography system (UPLC) capable of separating large numbers of metabolites found in complex biological samples. UPLC-MSE technique is used to compare metabolic profiles in the serum of a set of patients group from healthy to NASH.
- 1 UPLC/MS/MS: Combines liquid chromatography with mass spectrometry (a more complex technology). Includes tentative identification of the markers.
Data analysis and Report Writing
Multivariate statistical methods, e.g. PCA and PLS, are typically used to analysed the results obtained, providing a versatile approach to modeling, monitoring and prediction in complex encountered within the metabolomics discipline.
A list of biomarkers is also presented in the final report, together with the results of identification experiments where appropriate. On conclusion of the project, all raw data are made available to the client.
With this web OWL Genomics intends to provide users with information to better understand their health and their diagnosed illnesses. You should consult with a qualified
physician for diagnosis and for answers to your personal questions/circunstances.
Last update: 09/06/2010
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